Aim. To investigate the role of Coxsackie-adenovirus receptor (CAR) in inflammatory bowel disease (IBD). Background. CAR, a\ntype I transmembrane protein with functions in virus attachment, has been shown to be associated with epithelial tight junctions\n(TJs) and mediates cell adhesion, implying its potential roles in the pathogenesis of IBD. Methods and Materials. To determine\nthe effect of CAR in IBD using QPCR and Western blotting to determine the expression of CAD in TNF .. induced NCM460\nand SW480 cells and IBD tissues compared to control groups. Furthermore, TJs dysregulation, FITC-Dextran permeability assay,\nqRT-PCR, Western blot, and IF assessed the permeability in CAR overexpressed cells treated with TNF .. HE, qRT-PCR, Western\nblot, and IHC assay were used to assess the CAR overexpressed cells whether they have the effect to cure DSS induced ulcerative\ncolitis rat model in vivo. Result.We found CAR levels in human colon cell lines are significantly downregulated under the treatment\nof tumor necrosis factor-alpha .... Furthermore, overexpression of CAR markedly prevented TNF ... induced inflammatory\nresponse, TJs dysregulation, and permeability disruption (FITC-Dextran permeability assay) in cells. Consistentwith these findings\nin vitro, we found that CAR overexpression could suppress gut inflammation, attenuate the downregulation of TJ protein ZO-1 and\nOccludin, and limit the induction of barrier permeability in a DSS induced ulcerative colitis ratmodel in vivo. Together, our findings\nstrongly suggest that CAR could protect tight junctions and has an anti-inflammatory effect during the pathogenesis of IBD.Thus\nCAR may serve as a therapeutic target for the diagnosis and treatment of IBD.
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